Molecular Typing of Streptococcus pyogenes.
نویسندگان
چکیده
منابع مشابه
Typing of Streptococcus pyogenes strains using the phage profiling method
We recently developed a method that allows fast differentiation between Streptococcus pyogenes (GAS) strains. The method named phage profiling (PP) is based on a simple assumption that a regular PCR reaction with Taq polymerase and relatively short elongation time is not able to yield long DNA fragment, such as ~40-50 kb integrated prophage. Only fragments without any integrated DNA or short fr...
متن کاملSimplification of a locus-specific DNA typing method (Vir typing) for Streptococcus pyogenes.
We describe a simplification of a highly discriminatory molecular typing method, called Vir typing, for Streptococcus pyogenes (D. Gardiner, J. Hartas, B. Currie, J. D. Mathews, D. J. Kemp, and K. S. Sriprakash, PCR Methods Appl. 4:288-293, 1995). The procedure can be completed within a day, is reproducible, and can be applied directly to colonies growing on primary culture plates, allowing rap...
متن کاملStreptococcus pyogenes ( Group A Streptococcus )
Group A streptococcus (GAS) is synonymous with Streptococcus pyogenes, the only species within this group of β-hemolytic streptococci. GAS is one of the leading pathogenic bacteria that infects children and adolescents, and is associated with a wide spectrum of infections and disease states. Worldwide, there are estimated to be >600 million cases of GAS pharyngitis (“strep throat”) and >100 mil...
متن کاملMolecular epidemiology of sil locus in clinical Streptococcus pyogenes strains.
Streptococcus pyogenes (group A Streptococcus [GAS]) causes a wide variety of diseases, ranging from mild noninvasive to severe invasive infections. Mutations in regulatory components have been implicated in the switch from colonization to invasive phenotypes. The inactivation of the sil locus, composed of six genes encoding a quorum-sensing complex, gives rise to a highly invasive strain. Howe...
متن کاملMolecular Characterization of Streptococcus pyogenes Isolated from Children with Pharyngitis
Submit Manuscript | http://medcraveonline.com [1]. Streptococcal pharyngitis is most significant disease, and its peak incidence occurs in children, these infections impose the economic burden on the society [2]. The symptoms of streptococcal pharyngitis include a sore throat, pain in swallowing, fever, swollen, and tender lymph nodes in the neck and fatigue [3]. S. pharyngitis oftenly results ...
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ژورنال
عنوان ژورنال: Nippon Saikingaku Zasshi
سال: 1999
ISSN: 1882-4110,0021-4930
DOI: 10.3412/jsb.54.617